Oxidative phosphorylation by subcellular particles from yeast

Factors affecting oxidative phosphorylation by subcellular particles isolated from cotton seedling hypocotyls.

Properly prepared mitochondrial fractions from plant sources are capable of incorporating inorganic phosphate into high energy bonds upon the oxidation of members of the tricarboxylic acid cycle (3, 6, 7). Oxidative phosphorylation has been demonstrated in preparations from a wide variety of plant sources. This author reported that a high concentration of BSA3 in the grinding medium was necessa...

Oxidative Phosphorylation and Glycolate Oxidation by Particles from Spinach Leaves.

It has recently been reported (2) that oxidative phosphorylation occurs when organic acids of the citric acid cycle are supplied as substrates to particles isolated from the cotyledons of etiolated lupine seedlings. The ratios of the number of moles of orthophosphate consumed to the number of atoms of oxygen taken up (P/0 ratios) were found to be as high as those previously reported only for an...

Oxidative phosphorylation catalyzed by cytoplasmic particles isolated from malignant tissues.

Oxidative phosphorylation in cardiolipin-lacking yeast mitochondria.

The role of cardiolipin in mitochondrial energy transformation was studied by comparing oxidative phosphorylation in the Saccharomyces cerevisiae cardiolipin synthase null mutant crd1Delta, and in isogenic wild type. Oxygen consumption experiments and membrane potential kinetics during the phosphorylation cycle in isolated mitochondria indicated that the absence of cardiolipin causes only a mod...

Interaction of fluorescent probes with submitochondrial particles during oxidative phosphorylation.

Addition of several N-arylaminonaphthalenesulfonate dyes to submitochondrial particles from bovine heart resulted in an enhancement of fluorescence of from SOto 150-fold and a considerable shift in the wave length of maximum emission toward the ultraviolet. The results indicate 40 to 55 dyebinding sites per mg of submitochondrial particles. A preliminary search revealed that mitochondrial ATPas...